1 Basic DESeq2 results exploration

Project: DESeq2-4v9-analysis.

2 Introduction

This report is meant to help explore DESeq2 (Love, Huber, and Anders, 2014) results and was generated using the regionReport (Collado-Torres, Jaffe, and Leek, 2016) package. While the report is rich, it is meant to just start the exploration of the results and exemplify some of the code used to do so. If you need a more in-depth analysis for your specific data set you might want to use the customCode argument. This report is based on the vignette of the DESeq2 (Love, Huber, and Anders, 2014) package which you can find here.

2.1 Code setup

This section contains the code for setting up the rest of the report.

## knitrBoostrap and device chunk options
load_install('knitr')

## Warning: package 'knitr' was built under R version 3.4.3

opts_chunk$set(bootstrap.show.code = FALSE, dev = device)
if(!outputIsHTML) opts_chunk$set(bootstrap.show.code = FALSE, dev = device, echo = FALSE)

#### Libraries needed

## Bioconductor
load_install('DESeq2')
if(isEdgeR) load_install('edgeR')

## CRAN
load_install('ggplot2')
if(!is.null(theme)) theme_set(theme)
load_install('knitr')
if(is.null(colors)) {
    load_install('RColorBrewer')
}
load_install('pheatmap')
load_install('DT')
load_install('devtools')

## Warning: package 'devtools' was built under R version 3.4.3

## Working behind the scenes
# load_install('knitcitations')
# load_install('rmarkdown')
## Optionally
# load_install('knitrBootstrap')

#### Code setup

## For ggplot
res.df <- as.data.frame(res)

## Sort results by adjusted p-values
ord <- order(res.df$padj, decreasing = FALSE)
res.df <- res.df[ord, ]
res.df <- cbind(data.frame(Feature = rownames(res.df)), res.df)
rownames(res.df) <- NULL

3 PCA

## Transform count data
rld <- tryCatch(rlog(dds), error = function(e) { rlog(dds, fitType = 'mean') })

## Perform PCA analysis and make plot
plotPCA(rld, intgroup = intgroup)

## Get percent of variance explained
data_pca <- plotPCA(rld, intgroup = intgroup, returnData = TRUE)
percentVar <- round(100 * attr(data_pca, "percentVar"))

The above plot shows the first two principal components that explain the variability in the data using the regularized log count data. If you are unfamiliar with principal component analysis, you might want to check the Wikipedia entry or this interactive explanation. In this case, the first and second principal component explain 30 and 14 percent of the variance respectively.

4 Sample-to-sample distances

## Obtain the sample euclidean distances
sampleDists <- dist(t(assay(rld)))
sampleDistMatrix <- as.matrix(sampleDists)
## Add names based on intgroup
rownames(sampleDistMatrix) <- apply(as.data.frame(colData(rld)[, intgroup]), 1,
    paste, collapse = ' : ')
colnames(sampleDistMatrix) <- NULL

## Define colors to use for the heatmap if none were supplied
if(is.null(colors)) {
    colors <- colorRampPalette( rev(brewer.pal(9, "Blues")) )(255)
}

## Make the heatmap
pheatmap(sampleDistMatrix, clustering_distance_rows = sampleDists,
    clustering_distance_cols = sampleDists, color = colors)

This plot shows how samples are clustered based on their euclidean distance using the regularized log transformed count data. This figure gives an overview of how the samples are hierarchically clustered. It is a complementary figure to the PCA plot.

5 MA plots

This section contains three MA plots (see Wikipedia) that compare the mean of the normalized counts against the log fold change. They show one point per feature. The points are shown in red if the feature has an adjusted p-value less than alpha, that is, the statistically significant features are shown in red.

## MA plot with alpha used in DESeq2::results()
plotMA(res, alpha = metadata(res)$alpha, main = paste('MA plot with alpha =',
    metadata(res)$alpha))

This first plot shows uses alpha = 0.1, which is the alpha value used to determine which resulting features were significant when running the function DESeq2::results().

## MA plot with alpha = 1/2 of the alpha used in DESeq2::results()
plotMA(res, alpha = metadata(res)$alpha / 2,
    main = paste('MA plot with alpha =', metadata(res)$alpha / 2))

This second MA plot uses alpha = 0.05 and can be used agains the first MA plot to identify which features have adjusted p-values between 0.05 and 0.1.

## MA plot with alpha corresponding to the one that gives the nBest features
nBest.actual <- min(nBest, nrow(head(res.df, n = nBest)))
nBest.alpha <- head(res.df, n = nBest)$padj[nBest.actual]
plotMA(res, alpha = nBest.alpha * 1.00000000000001,
    main = paste('MA plot for top', nBest.actual, 'features'))

The third and final MA plot uses an alpha such that the top 500 features are shown in the plot. These are the features that whose details are included in the top features interactive table.

6 P-values distribution

## P-value histogram plot
ggplot(res.df[!is.na(res.df$pvalue), ], aes(x = pvalue)) +
    geom_histogram(alpha=.5, position='identity', bins = 50) +
    labs(title='Histogram of unadjusted p-values') +
    xlab('Unadjusted p-values') +
    xlim(c(0, 1.0005))

## Warning: Removed 2 rows containing missing values (geom_bar).

This plot shows a histogram of the unadjusted p-values. It might be skewed right or left, or flat as shown in the Wikipedia examples. The shape depends on the percent of features that are differentially expressed. For further information on how to interpret a histogram of p-values check David Robinson’s post on this topic.

## P-value distribution summary
summary(res.df$pvalue)

##    Min. 1st Qu.  Median    Mean 3rd Qu.    Max.    NA's 
##  0.0000  0.2432  0.5425  0.5223  0.8128  1.0000     258

This is the numerical summary of the distribution of the p-values.

## Split features by different p-value cutoffs
pval_table <- lapply(c(1e-04, 0.001, 0.01, 0.025, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5,
    0.6, 0.7, 0.8, 0.9, 1), function(x) {
    data.frame('Cut' = x, 'Count' = sum(res.df$pvalue <= x, na.rm = TRUE))
})
pval_table <- do.call(rbind, pval_table)
if(outputIsHTML) {
    kable(pval_table, format = 'markdown', align = c('c', 'c'))
} else {
    kable(pval_table)
}

Cut	Count
0.0001	84
0.0010	197
0.0100	591
0.0250	945
0.0500	1407
0.1000	2248
0.2000	3637
0.3000	5041
0.4000	6478
0.5000	7887
0.6000	9311
0.7000	10868
0.8000	12505
0.9000	14410
1.0000	16961

This table shows the number of features with p-values less or equal than some commonly used cutoff values.

7 Adjusted p-values distribution

## Adjusted p-values histogram plot
ggplot(res.df[!is.na(res.df$padj), ], aes(x = padj)) +
    geom_histogram(alpha=.5, position='identity', bins = 50) +
    labs(title=paste('Histogram of', elementMetadata(res)$description[grep('adjusted', elementMetadata(res)$description)])) +
    xlab('Adjusted p-values') +
    xlim(c(0, 1.0005))

## Warning: Removed 2 rows containing missing values (geom_bar).

This plot shows a histogram of the BH adjusted p-values. It might be skewed right or left, or flat as shown in the Wikipedia examples.

## Adjusted p-values distribution summary
summary(res.df$padj)

##    Min. 1st Qu.  Median    Mean 3rd Qu.    Max.    NA's 
##   0.000   0.642   0.846   0.753   0.944   1.000    6501

This is the numerical summary of the distribution of the BH adjusted p-values.

## Split features by different adjusted p-value cutoffs
padj_table <- lapply(c(1e-04, 0.001, 0.01, 0.025, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5,
    0.6, 0.7, 0.8, 0.9, 1), function(x) {
    data.frame('Cut' = x, 'Count' = sum(res.df$padj <= x, na.rm = TRUE))
})
padj_table <- do.call(rbind, padj_table)
if(outputIsHTML) {
    kable(padj_table, format = 'markdown', align = c('c', 'c'))
} else {
    kable(padj_table)
}

Cut	Count
0.0001	6
0.0010	30
0.0100	77
0.0250	115
0.0500	160
0.1000	307
0.2000	589
0.3000	878
0.4000	1246
0.5000	1746
0.6000	2388
0.7000	3174
0.8000	4655
0.9000	6565
1.0000	10718

This table shows the number of features with BH adjusted p-values less or equal than some commonly used cutoff values.

8 Top features

This interactive table shows the top 500 features ordered by their BH adjusted p-values. Use the search function to find your feature of interest or sort by one of the columns.

## Add search url if appropriate
if(!is.null(searchURL) & outputIsHTML) {
    res.df$Feature <- paste0('<a href="', searchURL, res.df$Feature, '">',
        res.df$Feature, '</a>')
}

for(i in which(colnames(res.df) %in% c('pvalue', 'padj'))) res.df[, i] <- format(res.df[, i], scientific = TRUE)

if(outputIsHTML) {
    datatable(head(res.df, n = nBest), options = list(pagingType='full_numbers', pageLength=10, scrollX='100%'), escape = FALSE, rownames = FALSE) %>% formatRound(which(!colnames(res.df) %in% c('pvalue', 'padj', 'Feature')), digits)
} else {
    res.df_top <- head(res.df, n = 20)
    for(i in which(!colnames(res.df) %in% c('pvalue', 'padj', 'Feature'))) res.df_top[, i] <- round(res.df_top[, i], digits)
    kable(res.df_top)
}

9 Count plots top features

This section contains plots showing the normalized counts per sample for each group of interest. Only the best 20 features are shown, ranked by their BH adjusted p-values. The Y axis is on the log10 scale and the feature name is shown in the title of each plot.

plotCounts_gg <- function(i, dds, intgroup) {
    group <- if (length(intgroup) == 1) {
        colData(dds)[[intgroup]]
    } else if (length(intgroup) == 2) {
        lvls <- as.vector(t(outer(levels(colData(dds)[[intgroup[1]]]), 
            levels(colData(dds)[[intgroup[2]]]), function(x, 
                y) paste(x, y, sep = " : "))))
        droplevels(factor(apply(as.data.frame(colData(dds)[, 
            intgroup, drop = FALSE]), 1, paste, collapse = " : "), 
            levels = lvls))
    } else {
        factor(apply(as.data.frame(colData(dds)[, intgroup, drop = FALSE]), 
            1, paste, collapse = " : "))
    }
    data <- plotCounts(dds, gene=i, intgroup=intgroup, returnData = TRUE)
    data <- cbind(data, data.frame('group' = group))
    main <- rownames(dds)[i]

    ggplot(data, aes(x = group, y = count)) + geom_point() + ylab('Normalized count') + ggtitle(main) + coord_trans(y = "log10")
}
for(i in head(ord, nBestFeatures)) {
    print(plotCounts_gg(i, dds = dds, intgroup = intgroup))
}

10 Reproducibility

The input for this report was generated with DESeq2 (Love, Huber, and Anders, 2014) using version 1.18.1 and the resulting features were called significantly differentially expressed if their BH adjusted p-values were less than alpha = 0.1. This report was generated in path /Users/vegardnygaard/prosjekter/rprojects/waaler-et-al-2018/troubleshooting using the following call to DESeq2Report():

## DESeq2Report(dds = dds, project = "DESeq2-4v9-analysis", intgroup = c("venngroup", 
##     "treatment"), outdir = "Analyse")

Date the report was generated.

## [1] "2018-11-09 14:12:40 GMT"

Wallclock time spent generating the report.

## Time difference of 1.285 mins

R session information.

## Session info ----------------------------------------------------------------------------------------------------------

##  setting  value                       
##  version  R version 3.4.2 (2017-09-28)
##  system   x86_64, darwin15.6.0        
##  ui       RStudio (1.1.383)           
##  language (EN)                        
##  collate  en_US.UTF-8                 
##  tz       <NA>                        
##  date     2018-11-09

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11 Bibliography

This report was created with regionReport (Collado-Torres, Jaffe, and Leek, 2016) using rmarkdown (Allaire, Xie, McPherson, Luraschi, et al., 2018) while knitr (Xie, 2014) and DT (Xie, 2018) were running behind the scenes. pheatmap (Kolde, 2018) was used to create the sample distances heatmap. Several plots were made with ggplot2 (Wickham, 2016).

Citations made with knitcitations (Boettiger, 2017). The BibTeX file can be found here.

[1] J. Allaire, Y. Xie, J. McPherson, J. Luraschi, et al. rmarkdown: Dynamic Documents for R. R package version 1.9. 2018. URL: https://CRAN.R-project.org/package=rmarkdown.

[1] C. Boettiger. knitcitations: Citations for ‘Knitr’ Markdown Files. R package version 1.0.8. 2017. URL: https://CRAN.R-project.org/package=knitcitations.

## No encoding supplied: defaulting to UTF-8.

[1] R. Kolde. pheatmap: Pretty Heatmaps. R package version 1.0.10. 2018. URL: https://CRAN.R-project.org/package=pheatmap.

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